Kuhner Shaker – SB50-X(OSB)

Orbital shaken bioreactor for single-use bags

The SB50-X is an orbital shaken bioreactor for the cultivation of human, mammalian and plant cells in a single-use bag. A working volume from 15 litres up to 50 litres for the SB50-X ensures easy scale-up from microtiter plates to shaking flasks and all the way up to 200 litres. The SB50-X can be used in research, process development or production.

The SB50-X was developed and tested in collaboration with ExcellGene SA (Monthey, Switzerland) and the Swiss Federal Institutes of Technology EPFL (Lausanne, Switzerland).

Performance characteristics

  • Large capacity (50L working volume)
    single use bioreactor system
  • Cultivation of human, mammalian and plant cells
  • Fast, simple set up
  • Easy scale-up
  • Online measurement of pH and DO

Bag

Single-use bag for fast and simple set up

The cylindrical vessel accepts a single-use bag. This bag requires no additional mixing device, enables quick set up times and eliminates elaborate cleaning and sterilising procedures. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The bag has built in optical sensors for pH and DO.

Orbital motion

The orbital motion of the SB50-X ensures efficient liquid mixing. It allows high oxygen transfer rates with low shear forces. Mechanical stress on the cells is reduced thanks to bubble-free surface gassing with almost no frothing. This motion is particularly suitable for microcarrier cultivation. The SB50-X uses the unique Kuhner direct drive which provides accurate control of the orbital speed.

Scale-up

Trouble free scale-up

The SB50-X fills a gap in the scale-up chain from microtiter plates all the way up to 200 litres. The consistent hydro-dynamics of shaken bioreactors provide reproducible cultivation conditions throughout the volume range. The culture from the SB50-X can also serve as preculture or inoculum for the SB200-X.

Control unit

Control unit and online measurement

The bespoke control unit, designed by Kuhner, uses the latest touch-screen technology and Kuhner Insight software for data recording, calibration, programming and controlling. Technical controls for 21 CFR Part 11 compliance are built into the software.

The SB50-X can be heated or cooled. Temperature ramps are easily programmed and processed.
Online measurement of each parameter (dissolved oxygen and pH-value in the medium) is available.

Pumping system

The control unit incorporates two small and one big peristaltic pump. This enables simple regulation of the pH value, feeding and the transfer of the culture media (scale-up or processing).

Gas mixing

The SB50-X has an integrated gas mixing device (FlowCon).
Whether stabilizing the pH with CO2, or preventing low dissolved oxygen concentrations, the gas mixer meets all user needs. Four mass flow controllers ensure highly accurate and reproducible gas mixing. An integrated pressure sensor prevents over pressure in the bioreactor by reducing the gas flow in the first step (1. limit) and shutting off the gas flow in the second step (2. limit). Step limits can be freely programmed by the user. Integrated air filters and back pressure valves ensure interference free and long operating times for the FlowCon.

Filter heater

A height adjustable filter heater, with up to two exhaust filters, is installed above the cylindrical vessel. Special emphasis has been placed on developing a user friendly unit. The filter heater combats any water vapour condensation in the outlet air which might cause the membranes to block. The temperature is monitored and controlled by Kuhner Insight software.

SMX1021

220 – 240 V / 50-60 Hz

SMX1023

110 – 120 V / 50-60 Hz

pH measurement

principle: optical chemosensor

Range

pH 5.5 – pH 8.5

Accuracy (chemosensor)

± pH 0.05 at pH 7 with one point calibration
± pH 0.10 at pH 7 with pre-calibration

Drift

< pH 0.005 per day

Temperature range

up to 50°C

 

DO measurement

principle: optical chemosensor

Range

0% – 100% DO

Accuracy (chemosensor)

± 0.1% O2 at 20.9% O2

Accuracy (system)

± 10% DO

Drift

< 0.015% O2 per day

Temperature range

up to 50°C

Capacity

2 exhaust filters

Temperature range

Ambient temperature up to 45°C,
monitored and controlled by Kuhner Insight software

 

SMX7110

Weight

120kg

Monitor

15” touchscreen monitor

Software

for monitoring, calibration, controlling

Monitoring

O2, pH, shaking speed, temperature

Calibration

pre-calibrated sensors in bag

Recalibration

one point recalibration is possible

Applicable

XP/Vista/Win7&8

GMP

21 CFR Part 11 compliance (audit trail)

2 small pumps (A+B)

Watson-Marlow 313VBM/D

Gear

10

Maximum Speed

100 rpm

Power

70 W

Rollers

3

Tube

1.6 mm wall tubing 

Delivery Pressure

max. 2bar


1.6 mm wall tubing
Bore diameter (mm)

Flow rates (ml/min at 100 rpm)

0.5

3.4

0.8

7

1.6

27

3.2

100

4.8

220

6.4

360

8.0

500

Number of mass flow controllers

up to 4

Ambient conditions

5-40 [°C], max. 85 [%rH]

Input

up to 4 gases, push in connector 6 [mm]
(air, oxygen,nitrogen, carbon dioxide)
(gas mixtures can also be connected)

Input pressure

1-2 bar

Output 

1, (tube fitting for tube ID 13 [mm])

Flow rate

0 – 20 [sL/min]

Accuracy

±1.5% from the end value

Pressure sensor

integrated, 0-400 [mbar], programmable
prevents overpressure in the connected vessel

Air filter

integrated, prevents blocking the mass flow controllers

Back pressure valve

integrated, prevents reflux into the mass flow controllers

Controlled filter
heating box

for the outlet filters, prevents blocking of the filters caused by condensation
(only air inlet and outlet with silicon tubing)

C-Flex material

for the tubing, enables the user to connect additional components via tube welding